ISOLATION AND CHARACTERIZATION OF CARBENDAZIM DEGRADING Trichoderma harzianum RIFAI MUTANTS

ITAMAR SOARES DE MELO, CÉLIA MARIA MAGANHOTTO DE S. SILVA, JANE L. FAULL

Resumo


Mutants of Trichoderma harzianum Rifai, obtained after ultraviolet (UV) light exposure, showed high resistant to the fungicide benomyl. A mutant (2B6) was capable of degrading carbendazim, other fungicide of the benzimidazole fungicide. This mutant degraded 41.5% of the molecule within five days. This and others mutants (2B1 and 2B2) presented variation in size and frequency of uni-nucleated and/or bi-nucleated spores compared to the wild type. Four primers generated RAPDs patterns that allowed the mutant to be differentiated from the wild-type. It is concluded that using UV mutagenization, it is feasible to obtain strains of T. harzianum with improved pesticide degradation ability.


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DOI: http://dx.doi.org/10.5380%2Fpes.v20i1.20452

Pesticidas: Revista de Ecotoxicologia e Meio Ambiente. ISSN:19839847